Abstract
Purpose: Endometriosis is defined as a common gynecologic and inflammatory disease. Transforming growth factor-beta 1 (TGF-β1) gene and its protein level might play a role in the pathogenesis of endometriosis. The present study aimed for the first time to assess the associations between endometriosis risk and −509 C/T (rs1800469) variant of the TGF-β1 gene as well as TGF-β1 mRNA expression in eutopic endometrium tissue of patients with and without endometriosis among a group of Iranian women.
Methods: Genotyping was carried out in 100 endometriosis patients (cases) with confirmed histological diagnosis of endometriosis and 197 non-endometriosis subjects (controls). The expression level of TGF-β1 mRNA was determined using the Real-Time PCR assay in 15 eutopic endometrium tissue of women with endometriosis and 15 healthy controls.
Results: There was a significant association for allele and genotype frequencies of the rs1800469 variant and endometriosis. No significant difference for the TGF-β1 expression was observed between eutopic endometrium of patients and the healthy group. Also, evaluation of TGF-β1 expression across the menstrual cycle showed the same level of TGF-β1 among case and control
subjects.
Conclusion: Our investigations indicated enough evidence for the effect of the TGF-β1 genetic variant on endometriosis risk in an Iranian population. Furthermore, we could not find any relations between TGF-β1 mRNA expression and susceptibility to endometriosis.
Keywords: TGF-β1; Gene expression; Variant; Endometriosis